Analyzing Data from a Splice Array Experiments
Abstract:
Splice-specific microarrays provide a basis to investigate
the effect of mutations and other factors on splicing
events in the creation of mature mRNA. This talk will
illustrate various statistical designs and analysis
issues from a study aimed at detecting differential gene
expression between selected spliceosome mutants. The data features an
unbalanced, nested design with minimal degree of replication. I will
begin the talk with a brief overview of the splice array technology and
discuss potential methods for synthesizing results from various
approaches. The design of these arrays also provide a platform for
comparing the performance of different normalization methods.
Slides: Download [pdf file]